Flow Cytometry Shared Resource Manager - 122238

The Moores Cancer Center (MCC) is one of just 53 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between UCSD: encompassing 28 departments, 6 schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health, Jacobs School of Engineering, School of Biological Sciences & the School of Physical Sciences), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University (SDSU), and the basic and translational research of the La Jolla Institute of Immunology (LJI).

These various programs and units are all dedicated to fulfilling MCC's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs. As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. MCC follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.

MCC's mission is to transform cancer care in our catchment area and beyond by driving exceptional scientific discoveries and innovations in prevention, detection, care, and training. MCC will make a global impact on improving health by reducing cancer burden through accelerated discovery and translation, compassionate and interdisciplinary care, education, and community engagement, with the foundation of our core values - Excellence, Innovation and Risk-Taking, Collaboration, Diversity and Service.

Under general supervision, manage and perform technical and administrative operations for the UCSD Moores Cancer Center Flow Cytometry Shared Resource.

Responsibilities include:

1. Providing Shared Resource users with consultations, training, scientific guidance, protocols, project ideas, and troubleshooting for flow cytometry and cell sorting experiments.
2. Operate a BD FACSymphony A1 flow cytometer and FACSymphony S6 cell sorter to conduct and assist with experiments using biological tissues and cells.
3. Instruct and direct Shared Resource users on flow cytometry data analysis and interpretation.
4. Collect, process, and analyze flow cytometry data for quantitative analysis using advanced software.
5. Maintain Shared Resource records and databases relating to instrument scheduling, usage, maintenance, and supply inventories.
6. Manage and maintain computer workstations, flow cytometry data storage and distribution.
7. Build and maintain a collaborative Shared Resource environment with the highest regard for customer service and satisfaction.
8. Stay apprised of technological advances and new instrumentation for flow cytometry and cell sorting to ensure the Shared Resource is equipped with leading edge technologies.

• Advanced practical knowledge and experience operating a 16- and 23-color BD platform digital flow cytometer and cell sorter in a biosafety cabinet.

• In depth theoretical and practical knowledge of and experience with multiparameter flow cytometry, cell sorting and data analysis.

• Demonstrated experience, expertise and skill using a 5-laser BD FACSymphony S6 cell sorter in a biosafety cabinet.

• Strong communication skills and track record of collaboration needed to interact professionally and effectively in the work environment. Ability to read, comprehend, and discuss research materials. Ability to write, edit and proofread research results.

• Skill and patience teaching, training and explaining difficult concepts to diverse users with different skill and experience levels. Track record of providing exceptional customer service and user experience.

• Advanced skill and experience in preparing live and fixed cell preparations for flow cytometry.

• Ability to independently advise and troubleshoot experiments and protocols for investigators who want to analyze or sort cell populations based on up to 25 distinct parameters.

• Theoretical and practical knowledge of biology and experimental techniques including immunocytometry, selective staining, standard staining of tissue and cells for flow cytometry.

• Ability to effectively interface with dozens of investigators from various UCSD laboratories who wish to access the MCC Flow Cytometry Shared Resource.

• Demonstrated ability in data management, analysis, interpretation, and description of scientific data in a publication format reports.

• Advanced knowledge of and experience with FACSDiva, FlowJo, MS Word, Excel, PowerPoint, Adobe Photoshop, Illustrator, and Graphpad PRISM software.

• Knowledge of Lab Safety Manual and Environmental Health and Safety rules and regulations with respect to lasers, lab safety, biosafety and chemical waste disposal.

• Experience in using Excel spreadsheets and PowerPoint to present quantification data from experimental results.

• Employment is subject to a criminal background check.