Staff Research Associate 2 (SRA 2 NEX)

The Hagerman Laboratory within the Department of Biochemistry and Molecular Medicine uses molecular techniques to characterize neurological disorders related to the FMR1 gene, specifically, fragile X syndrome (FXS) and fragile X-associated tremor/ataxia syndrome (FXTAS). Principal effort continues to be directed to the molecular characterization of gene expression and cellular pathways that underlie the pathogenesis of these disorders. Relevant fundamental techniques will place heavy reliance on human cell culture with fibroblasts and iPSC-derived neuronal cells; such techniques will include DNA, RNA, and protein extraction; qRT-PCR; TR-FRET; Western blot analysis; immunostaining; high-content imaging; mRNA- and miRNA-seq preparation; bioinformatics analysis; and both analytical and preparative flow cytometry. Additional, ongoing assay development will include TMT-MS and CGG-repeat allele typing. Continued development and optimization of assays will be pursued as needed to further the research goals of the laboratory. Research in the lab also relies heavily on the processing of both human and non-human (mammalian) brain tissues and blood samples. Importantly, the position requires the ability to conduct experiments and troubleshoot with minimal supervision, to interpret and document results, and to effectively communicate those results orally and in writing.

Apply By Date: 10/30/2023 at 11:59pm

Minimum Qualifications

• Minimum of bachelor's degree in genetics, molecular biology, or related field
• Minimum 2 years of relevant wet-lab molecular genetics research experience post bachelor's degree (does not include classroom work).
• Demonstrated ability to develop and adapt assays with some guidance
• At least two (2) years of primary/independent experience in human and general eukaryotic cell culture.
• Expertise in a broad range of skills to conduct experiments in mammalian genetics: PCR, qRT-PCR, purification of DNA, RNA and protein, immunoblotting (Westerns), and immunostaining.
• Experience in molecular cloning
• Experience with analysis and presentation of data in written and spoken reports in English
• Proficiency in effectively using personal computer and software, including but not limited to MS Word and Excel.
• Accurate and precise writing, printing, and typing skills in English.
• High attention to detail
• Excellent interpersonal and communication skills to communicate in a pleasant, tactful, and effective manner and to handle difficult, sensitive, and/or confidential issues with others.
• Ability to learn and follow ethical principles and federal and state regulations governing the participation of human subjects in biomedical research, as well as rules and regulations regarding lab safety.

Preferred Qualifications

• Experience in FMR1-related disorders or other neurodevelopmental/neurodegenerative disorders
• Experience in the culture of iPSCs and iPSC-derived neuronal cells
• Experience with analytical and preparative cell sorting
• Coding experience (R, python, perl, or other software for bioanalysis)
• Familiarity with sample preparation and data analysis for mass spectrometry and/or mRNA/miRNA-seq (or other big data projects)
• 3 or more years of relevant wet-lab molecular genetics research experience post bachelor's degree (does not include classroom work)
• Experience with FRET assay to detect and characterize protein expression in patient samples
• Experience with the design and use of ELISA assays for protein quantification
• Experience and skill with mouse brain dissection and experience working with both human and mouse brain tissues
• Experience in high-content imaging

Department Description

The Department of Biochemistry and Molecular Medicine's mission is to conduct world-class research in biochemistry and molecular medicine, to excel in undergraduate, graduate, and medical education, and to serve the university through leadership in forums committed to graduate and professional school admissions and curriculum. Research in the Department of Biochemistry and Molecular Medicine addresses the fundamental molecular aspects of cell biology, gene expression, cancer biology, membrane biology, glycobiology, neurobiology, muscle physiology, human genetics, chemical and structural biology, molecular imaging, and drug development. In addition to innovative research activities, faculty are involved in the teaching and training of medical and doctoral students.

The faculty of the Department of Biochemistry and Molecular Medicine maintain laboratories in the Davis Campus at Tupper Hall and in the Department of Chemistry. In Sacramento, the Department maintains laboratories at the Oak Park Research Building, UC Davis Cancer Center, and MIND Institute. The Department of Biochemistry and Molecular Medicine strives to build and maintain a culture and climate based on mutual respect and caring. We recognize and cherish the richness contributed to our lives by our diversity. We further recognize the right of every individual to think, speak, express and debate any idea. We support and uphold the UC Davis Principles of Community.

Position Information

• Salary or Pay Range: $2243.20 - $3608.80
• Salary Frequency: Non-Exempt
• Salary Grade: 108
• Payroll Title: SRA 2 NEX
• Number of Positions: 1
• Appointment Type: Career
• Percentage of Time: 100
• Shift Hours: 08 Hours
• Location: Tupper
• Union Representation: Yes
• Benefits Eligible: Yes

Benefits

Outstanding benefits and perks are among the many rewards of working for the University of California. UC Davis Health offers a full range of benefits, resources and programs to help you bring your best self to work, as well as to help you and your family achieve your health, wellness, financial and career goals. Learn more about the benefits below and eligibility rules by visiting our handy and our

• If you are represented by a union, benefits are negotiated between the University of California (UC) and your union and finalized in a contract. Read your bargaining unit's employment contract, stay abreast of current negotiations and learn about collective bargaining at UC:
• High quality and low-cost medical plans to choose from to fit your family's needs
• UC pays for Dental and Vision insurance premiums for you and your family
• Extensive leave benefits including Maternity, Paternity, Family & Medical Leave
• Paid Holidays annually as stipulated in the UC Davis Health Policies or Collective Bargaining Agreement.
• Paid Time Off/Vacation/Sick Time as stipulated in the UC Davis Health Policies or Collective Bargaining Agreement.
• Paid time off for professional development as stipulated in the UC Davis Health Policies or Collective Bargaining Agreement.
• Continuing Education (CE) allowance and Education Reimbursement Program as stipulated in the UC Davis Health Policies or Collective Bargaining Agreement.
• Access to free professional development courses and learning opportunities for personal and professional growth
• Wellness and on-site Employee Assistance Program including access to free mental health services
• Supplemental insurance offered including additional life, disability, short/long term disability, pet insurance and legal coverage
• Public Service Loan Forgiveness (PSFL) Qualified Employer & Student Loan Repayment Assistant for qualified roles
• Retirement benefit options for eligible roles including Pension and other Retirement Saving Plans. More information on our retirement benefits can be found
• UC Davis cares about building a community, which is why we provide resources to enhance as well as (ERGs) to support our staff

Physical Demands and Work Environment

• Carrying - up to 30 pounds
• The position requires operating and maintaining laboratory equipment. Also, (Elements Exposed to) - Some work will involve the use of viral vectors and viruses for cell transformation (EBV; AAV; lenti), and human and non-human mammalian cells and tissues, including blood and blood products. Employee must work in accordance with required laboratory and University safety procedures, including participation in required training (e.g., bloodborne pathogens, hazardous waste, and shipping hazardous material).

Special Requirements

• This position is a critical position and subject to a background check and drug screen. Employment is contingent upon successful completion of background investigation including criminal history, identity checks and drug screen.
• As a condition of employment, you will be required to comply with the University of California , as may be amended or revised from time to time. Federal, state, or local public health directives may impose additional requirements.

Diversity, Equity, Inclusion and Belonging

At UC Davis, we're solving life's most urgent challenges to bring a fuller, healthier, and more resilient world within reach. We grow from every challenge we take on and we don't just maintain - we improve. We recognize that creating an inclusive and intellectually vibrant organization means understanding and valuing both our individual differences and our common ground. The most comprehensive solutions come from the most diverse minds, and you belong here.

As you consider joining UC Davis, please explore our , our and for research and education, and our latest efforts to . The advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy.

Because we want you to be seen, our recruiting process at UC Davis fosters authenticity, diversity, and inclusion. Studies have shown that some people may not apply to jobs unless they meet every single qualification. Each unique role at UC Davis has a set of requirements and you could be perfect for this role, or you could be perfect for the next role! Don't meet all the requirements? We still encourage you to apply! #YouBelongHere