Minimum Salary
US-MA-Worcester
Job Location
3 weeks ago(7/29/2024 5:27 PM)
Requisition Number |
2024-46501
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# of Openings |
1
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Posted Date |
Day
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Shift |
Exempt
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Overview
POSITION SUMMARY: Under the direction of the Principal Investigator (PI) or designee, the Research Associate IV designs and performs a variety of complex research experiments with a high degree of independence. This position will contribute significantly to the management of the research program and will be involved in multiple aspects of the research projects and lab maintenance activities.
Responsibilities
Qualifications
REQUIRED QUALIFICATIONS:
- Bachelor's degree in Biological or Natural Science and 5+ years of relevant research experience or a related Master's degree with 3+ years of relevant research experience.
- Knowledge of Microsoft Office products
- Strong ability to analyze complex visual data in a quantitative and objective manner
- Ability to work in a team environment
PREFERRED QUALIFICATIONS:
- T cell responses to virus-specific immunology and heterologous immunity
- T cell sorting, TCR sequencing, T cell phenotyping
- BD-LSRII and BD-FACSymphony A5-SE cell analyzer
- Working expertise in BCL-2 laboratory
- Real-time PCR technique
- Experience and expertise fields: virology, immunology, microbiology, molecular biology
- Experience with PBMC isolation from human blood, CD4/CD8T cell/B cell sorting, HLA-typing, FACS staining, ELISPOT Assays, proliferation assays, ICS production Assay of T cells, NK and Treg cells.
- Skilled in Ag-specific phenotyping of CD4/CD8 and B cells; Viral load quantification in PBMC, nasal-oral washes and sputum; Genomic DNA, RNA isolation and amplification
- Skilled in Ab-subtyping/profiling; Setting up short term T cell cultures; TCR VA/VB / TCR V Gammadelta repertoire analysis
- Skilled in Freezing-thawing T-B cells, plasma and serum; Quantification of cytotoxic T cells by Chromium 51 release assay; Using GC 1000 irradiator
- Experience with T2, 721.221 and MLA-144 cell lines; Preparing MHC-Class I tetramers with different streptavidin conjugates
- Expertise on using Prism Graph Pad 10 and FlowJo 10 programs
Additional Information
Under the direction of the Principal Investigator, or designee SUPERVISION EXERCISED: Supervise aides, technicians, and associates ENVIRONMENTAL WORKING CONDITIONS: Wet chemistry laboratory, and may require appropriate contact with biohazards, radionuclides, toxins, animals, and human specimens
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